Stable Target-Expressing Cell Lines
We offer target and biomarker screening services for industry and academics providing you with cell lines expressing your target gene of choice. So far, we have generated
- 250 cDNA expression vectors,
- 850 stable recombinant cell lines for the expression of
- 200 targets, using
- 150 maternal cell lines or primary cells.
- Custom cell models using CRISPR/Cas9.
We support your target validation by:
- Cloning your gene of interest into a suitable expression vector
- Selection of promoters of different strengths
- Lentiviral transduction of a cell line / primary cell type of your choice
- Selection of stable recombinant cell lines (polyclonal or monoclonal), validated by gene expression analysis at the mRNA / protein level
- Characterisation with respect to target protein localisation by immunofluorescence microscopy or FACS analysis
- Full range of functional characterisations (e.g. cell proliferation, induction of apoptosis, metabolism; please inquire for further assays)
High-content analysis of cellular phenotypes
Combine the Chromobody® technology with your gene of interest!
For further information and pricing please contact
- Double expression of your target gene and of an indicative Chromobody® in a single cell line
- High-content analysis for monitoring cell cycle progression, nuclear morphology, cytoskeletal integrity
Dr. Frank Weise.