Genome Editing by CRISPR/Cas9
Based on our long-standing expertise in generating customised cell lines, we are now also employing the CRISPR/Cas9 genome editing approach. Full freedom to operate (FTO) is ensured through a set of license agreements (please see our agreement with ERS Genomics).
The CRISPR/Cas9 technology can also be used for target validation, analogous to RNAi mediated target silencing.
Introduction of specifically site-directed double stand breaks applying the CRISPR/Cas9 technology
Generation of target-defective cell lines
Larger deletions by two double strand breaks
Insertion of specific mutations or extensions into the DNA locus with ectopic repair templates
Generation of synthetic human hiPSC disease models