Custom Cell Lines | NMI TT Pharmaservices

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Person

NMI-TT Contact Person

Dr. Frank Weise

+49(0)121-51530 56




NMI TT Ion Channel Services

Customised Cell Lines

We offer target and biomarker screening services for industry and academics providing you with cell lines expressing your target gene of choice. So far, we have generated

  • 250 cDNA expression vectors,
  • 850 stable recombinant cell lines for the expression of
  • 200 targets, using
  • 50 maternal cell lines or primary cells.

Target validation

    We support your target validation by:
  • Cloning your gene of interest into a suitable expression vector
  • Selection of promoters of different strengths
  • Lentiviral transduction of a cell line / primary cell type of your choice
  • Selection of stable recombinant cell lines (polyclonal or monoclonal), validated by gene expression analysis at the mRNA / protein level
  • Characterisation with respect to target protein localisation by immunofluorescence microscopy or FACS analysis
  • Full range of functional characterisations (e.g. cell proliferation, induction of apoptosis, metabolism; please inquire for further assays)

High content analysis of cellular phenotype
Combine the Chromobody® technology with the expression of your gene of interest!

  • Double expression of your gene of interest and of an indicative Chromobody┬« in a single cell line
  • High content analysis for monitoring cell cycle progression, nuclear morphology, cytoskeletal integrity
For further information and pricing please contact
Dr. Frank Weise

 

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NMI TT is a subsidiary of NMI