Calcium Imaging | NMI TT Pharmaservices

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Electrophysiology

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NMI-TT Contact Person

Dr. Udo Kraushaar

+49 7121 51530 851


Zeiss Cell Explorer

 
 


Hamamatsu FDSS/uCell

Imaging

Besides classical electrophysiological approaches we offer life cell imaging based techniques in order to monitor cell-cell interactions / analyze compound induced effects on intracellular ion homeostasis.

Monitoring the dynamic changes of the intracellular Ca2+ concentration enables the understanding of cardiac function including inotropic effects and the occurrence of proarrhythmic events.

Two technologies are available for detailed investigations of the intracellular Ca2+ concentrations:

  • Zeiss Cell Explorer with attached Yokogawa spinning disk for rapid confocal imaging offers subcellular resolution with sampling frequencies up to 80 Hz
  • Hamamatsu FDSS/Cell is our higher throughput working horse with parallel recordings in 96- or 384-well format at a sampling frequency of up to 120 Hz.



Confocal Ca2+ imaging of cultivated, spontaneously beating cardiomyocytes using the Zeiss Cell Observer system. Top: Time series of false-color coded Ca2+ dependent fluorescence of Cal520-AM loaded cardiomyocytes. Excitation leads to an transient intracellular Ca2+ increase, visualized by a color shift from blue via green to red. Bottom: Fluorescence intensity-over time trace of above shown recording. Numbers indicate time points of the images.


Intracellular Ca2+ Imaging of spontaneously beating cardiomyocytes using the higher throuput Hamamatsu FDSS/uCell system. A: Positive inotropic effect by Salbutamol. Application of this beta2-sympathomimetic drug results in a concentration-dependent increase of intracellular Ca2+ concentration during each beat. B: The Ca2+ channel Inhibitor Diltiazem both reduces Ca2+ transient amplitude and duration,, resulting in negative inotropicity. C: Application of the potent hERG channel inhibitor Dofetilide results in the detection of proarrhythmic behavior in the investigated cardiomyocytes.

 

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