DigiWest Protein Profiling Services | NMI TT Pharmaservices

home contact company.overview news sitemap print privacy

Protein Profiling

Contact

NMI-TT Contact Person

Dr. Markus Templin

+49 7121 51530 828


Download Flyer










How DigiWest works
How DigiWest works 

DigiWest Protein Profiling Services

Imagine you get data from 80, 300, or even 800 total & phospho proteins per sample. With DigiWest you can!

New! In addition to our fully customizable list of analytes, we have also developed pre-defined pathway panels. Just contact us to
get the list.

    DigiWest:
  • Is a novel proprietary immunoassay technology
  • Combines the robustness of Western blotting with the power of Luminex® bead-based multiplexing
  • Allows for quantification of up to 800 analytes from ~60 µg of protein
  • Is fully customizable, based on a list of >1,000 antibodies (and validated antibody lists for human, mouse, rat, dog, minipig, cyno)
  • Includes >260 post-translational modifications incl. phospho-epitopes
  • Enables comprehensive analyses from limited samples (e.g. LCM)
  • Is superior to MassSpec, needs less material & no enrichment
  • Represents a versatile new platform for pathway profiling, drug MoA studies, lead characterization, predictive tox and biomarker screening
  • Is being offered on a fee-for-service basis


Case Study 1: DigiWest for Biomarker Discovery

Analysis of mechanisms and biomarkers of Platinum resistance

  • A set of 24 fresh frozen tumor specimens from relapsed vs cured platinum-treated ovarian cancer patients was analysed by DigiWest, using 466 antibodies (total and phospho proteins) covering various cell signalling pathways.
  • DigiWest protein signatures distinguished platinum resistant vs sensitive patients, and revealed 8 biomarker candidates.

Case Study 2: DigiWest for Lead Characterization

Mode-of-action study of compounds in comparison to reference drugs

  • Calu1 cells were treated with 1 MEK inhibitor vs 1 PI3K inhibitor vs 2 experimental lead compounds (data not shown) vs DMSO. Cell samples were analysed by DigiWest, using 156 selected antibodies (total and phospho proteins) covering different signal transduction pathways. 
  • DigiWest yielded distinct signatures for each compound, and allowed in-depth characterization of lead compounds.


Case Study 3: DigiWest in PD3DTM Tumor Cell Models

Protein profiling for characterization of primary tumor samples 

  • In collaboration with cpo cellular phenomics & oncology GmbH, primary patient-derived 3D tumor cell cultures (PD3Ds) were profiled by DigiWest, focusing on 122 antibodies for total and phospho proteins involved in a range of signaling pathways. 
  • DigiWest revealed pathway activation patterns that explained differences in the PD3Ds' drug responses as measured by cpo.


Case Study 4: DigiWest in Multiple Species

Multiplex protein profiling for toxicology and in vivo pharmacology 

  • A DigiWest panel of >700 antibodies previously validated in human and mouse materials was tested in protein lysates from liver and kidney samples of rat, dog, minipig and cynomolgus origin. 
  • This comprehensive validation project has yielded hundreds of antibodies for DigiWest and Western blot studies, thus enabling protein profiling in toxicology and in vivo pharmacology.



Peer-Reviewed Publications

Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, Joos TO, Kling S, Poetz O, Fehm T, Neubauer H, Kuster B, Templin MF. A bead-based western for high-throughput cellular signal transduction analyses. Nature Communications 2016.

Augustin I, Dewi DL, Hundshammer J, Erdmann G, Kerr G, Boutros M. Autocrine Wnt regulates the survival and genomic stability of embryonic stem cells. Science Signaling 2017.

Menck K, Sönmezer C, Worst TS, Schulz M, Dihazi GH, Streit F, Erdmann G, Kling S, Boutros M, Binder C, Gross JC. Neutral sphingomyelinases control extracellular vesicles budding from the plasma membrane.
J Extracell Vesicles 2017
.

Mishra M, Tiwari S, Gomes AV. Protein purification and analysis: next generation Western blotting techniques. Expert Rev Proteomics 2017.

Kissel M, Berndt S, Fiebig L, Kling S, Ji Q, Gu Q, Lang T Hafner FT, Teufel M, Zopf D. Antitumor effects [...] in preclinical models of hepatocellular carcinoma. Oncotarget 2017.

De Boussac H, Gondeau C, Briolotti P, Duret C, Treindl F, Roemer M, Fabre JM, Herrero A, Ramos J, Maurel P, Templin M, Gerbal-Chaloin S, Daujat-Chavanieu M. Epidermal Growth Factor represses CAR expression in primary hepatocytes and favors regulation by Pregnane X Receptor.
Drug Metab Dispos 2017
.

Lomnytska M, Pinto R, Becker S, Engström U, Gustafsson S, Björklund C, Templin M, Bergstrand J, Xu L, Widengren J, Epstein E, Franzen B, Auer G. Platelet protein biomarker panel for ovarian cancer diagnosis. Biomarker Research 2018.

Mehul B, Perrin A, Grisendi K, Galindo AN, Dayon L, Menigot C, Rival Y, Voegel JJ. Mass spectrometry and DigiWest technology emphasize protein acetylation profile from Quisino-treated HuT78 CTCL cell line. Journal of Proteomics 2018.

Sikorski K, et al. A high-throughput pipeline for validation of antibodies. Nature Methods 2018.


Other References

Graham H. DigiWest: Reimaging Western blotting on the proteomic scale. Luminex Blog 2016.

Couvillon A, Templin MF. Cross-validation of antibodies using DigiWest. LabRoots Video 2016.

Evans J. A blot on the beads: Western blotting with a bead-based immunoassay. Electrophoresis 2016.

How to find the missing link between genotype and phenotype.
Labiotech 2017.

Sachse C, Regenbrecht CRA, Templin MF. Beyond Genomics. DigiWest-Technologie: Multiplex-Analyse von Proteinen und Phosphoproteinen. LABO 2017.

Pearson S. Screening innovations and 3D cell culture assay developments showcased at ELRIG. GEN 2017.

Singh G. The future of now - how the science of today shapes tomorrow. ScienceExchange Blog 2018.

Graham H. Drop the blot: Parallel quantification of up to 800 proteins. Luminex Blog 2018.

Erdmann G, et al. Towards 3Rs: Multiplex protein profiling from minipig, cyno, dog and rat samples, to get the most our of preclinical safety studies. Ellegaard Newsletter 2018.

Sachse C, Templin M. Multiplexing Western blots: More data, less sample. GEN 2018.

DigiWest: Multiplex profiling of proteins for biomarker discovery. LabsExplorer 2018.

 

Linked in profile

NMI TT is a subsidiary of NMI