Up to 800 western blots in one go.
Total and phospho proteins.
About DigiWest®
DigiWest® is an advanced multiplex protein profiling service for in-depth pathway activity analyses in drug development and biomarker discovery. Save time, go beyond genomics, use less sample, and save lab resources - look at hundreds of total and phosphoproteins in one go!
Applications
DigiWest® can be used for a wide range of research, from compound mode-of-action studies to biomarker discovery on the protein level. DigiWest® studies are fully customizable, for profiling of one or more signaling pathways.
Samples
Just 20-60 μg of total protein is needed for DigiWest® protein profiling, enabling highly parallelized protein profiling analyses from 2D/3D cells, organoids, xenografts, PDX, tissues, and tissue sections (fresh-frozen or FFPE).
Analytes
80 to 800 proteins can be studied in one DigiWest®. And the choice is up to you - we offer a selection of 1,200+ analytes (including 300+ phospho-epitopes), which can be picked manually or from 50+ different signaling pathway panels.
About Us
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Our Story
We help you achieve success by providing high-quality and state-of-the-art services. Founded in 2002 as a subsidiary of NMI Natural and Medical Sciences Institute at the University of Tübingen, we are a trusted non-clinical CRO partner for pharma, biotech, and academia.
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Our Services
We are experts in protein profiling services. As an innovation orientated CRO company, we constantly develop new methods or improve the existing ones. DigiWest® is one of our proprietary services. We combine innovative thinking with a personal approach to our clients.
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Contact us
Email: berlin@nmi-tt.de
Phone: +49 173 751 0585
NMI TT Pharmaservices
Campus Berlin-Buch, D79, 1. OG
Robert-Rössle-Str. 10
13125 Berlin
Germany
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Publications
Pharma Publications
- Eli Lilly: Blosser et al. A pan-cancer transcriptome analysis identifies replication fork and innate immunity genes as modifiers of response to the CHK1 inhibitor prexasertib. Oncotarget 2020
- Bayer: Christian et al. The novel dihydroorotate dehydrogenase (DHOH) inhibitor BAY2402234 triggers differentiation and is effective in the treatment of myeloid malignancies. Leukemia 2019
- Galderma: Mehul et al. Mass spectrometry and DigiWest technology emphasize protein acetylation profile from [...] treated HuT78 CTCL cell line. J of Proteomics 2018
- Bayer: Kissel et al. Antitumor effects of [...] in preclinical models of hepatocellular carcinoma. Oncotarget 2017
Our Publications
- With the University of Tübingen: Marzi J, et. al. Noninvasive Physical Plasma as Innovative and Tissue-Preserving Therapy for Women Positive for Cervical Intraepithelial Neoplasia. Cancers (Basel) 2022.
- With the University of Tübingen: Holl M, et. al. Cell Type-Specific Anti-Adhesion Properties of Peritoneal Cell Treatment with Plasma-Activated Media (PAM). Biomedicines 2022.
- With the Charles Uni: Skoda J, et. al. Off-target lipid metabolism disruption by the mouse constitutive androstane receptor ligand TCPOBOP in humanized mice. Biochemical Pharmacology 2022.
- With DKFZ: Bamberg L V, et. al.Targeting euchromatic histone lysine methyltransferasessensitizes colorectal cancer to histone deacetylase inhibitors. Intern J of Cancer 2022.
- With the NMI: Billing F, et. al. Altered Proinflammatory Responses to Polyelectrolyte Multilayer Coatings Are Associated with Differences in Protein Adsorption and Wettability. Applied Mater Interfaces 2021.
- With the NMI: Kling S, et. al.Characterization of hepatic zonation in mice by mass-spectrometric and antibody-based proteomics approaches. Bio Chem 2021.
- With the NMI : Fink S, et. al. Multiplexed Serum Antibody Screening Platform Using Virus Extracts from Endemic Coronaviridae and SARS-CoV-2. ACS Infect Dis 2021.
- With the University Hospital Münster: Pogatzki-Zahn E, et. al. A proteome signature for acute incisional pain in dorsal root ganglia of mice. Pain 2021.
- With the University of Tübingen: Zbinden A, et. al. Nidogen-1 Mitigates Ischemia and Promotes Tissue Survival and Regeneration. Advanced Science 2020.
- With the University of Edinburgh: Shukla R, et. al. Activation of transcription factor circuity in 2i-induced ground state pluripotency is independent of repressive global epigenetic landscapes. Nucleic Acids Research 2020.
- With Charité Berlin: Bockmayr T, et. al. Multiclass cancer classification in fresh frozen and formalin-fixed paraffin-embedded tissue by DigiWest multiplex protein analysis. Laboratory Investigation 2020.
- With Freie Universität Berlin: Jevtic M, et. al. Impact of intercellular crosstalk between epidermal keratinocytes and dermal fibroblasts on skin homeostasis. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 2020
- With BfR: Treindl et al. Array-based Western-blotting reveals spatial differences in hepatic signaling and metabolism following CAR activation. Archives of Toxicology 2020
- With Trinity College Dublin: Inder et al. Multiplex profiling identifies clinically relevant signaling proteins in an isogenic prostate cancer model of radioresistance. Scientific Reports 2019
- With the University of Düsseldorf: Naskou et al. EZH2 loss drives resistance to carboplatin and paclitaxel in serous ovarian cancers expressing ATM. Molecular Cancer Research 2019
- With DKFZ: Zhan et al. MEK inhibitors activate Wnt signaling and induce stem cell plasticity in colorectal cancer. Nature Communications 2019
- With CELLphenomics: Schumacher et al. Heterogeneous pathway activation and drug response modeled in colorectal-tumor-derived 3d cultures. PLoS Genetics 2019
- With DKFZ: Augustin et al. Autocrine Wnt regulates the survival and genomic stability of embryonic stem cells. Science Signaling 2017
- With the University of Göttingen: Menck et al. Neutral sphingomyelinases control extracellular vesicles budding from the plasma membrane. J Extracell Vesicles 2017
- With the University of Tübingen: Treindl et al. A bead-based western for high-throughput cellular signal transduction analyses. Nature Communications 2016