Up to 800 western blots in one go.
Total and phospho proteins.

About DigiWest®


DigiWest® is an advanced multiplex protein profiling service for in-depth pathway activity analyses in drug development and biomarker discovery. Save time, go beyond genomics, use less sample, and save lab resources - look at hundreds of total and phosphoproteins in one go!


DigiWest® can be used for a wide range of research, from compound mode-of-action studies to biomarker discovery on the protein level. DigiWest® studies are fully customizable, for profiling of one or more signaling pathways.


Just 20-60 μg of total protein is needed for DigiWest® protein profiling, enabling highly parallelized protein profiling analyses from 2D/3D cells, organoids, xenografts, PDX, tissues, and tissue sections (fresh-frozen or FFPE).


80 to 800 proteins can be studied in one DigiWest®. And the choice is up to you - we offer a selection of 1,200+ analytes (including 300+ phospho-epitopes), which can be picked manually or from 50+ different signaling pathway panels.


About Us

Our Story

We help you achieve success by providing high-quality and state-of-the-art services. Founded in 2002 as a subsidiary of NMI Natural and Medical Sciences Institute at the University of Tübingen, we are a trusted non-clinical CRO partner for pharma, biotech, and academia.  

Our Services

We are experts in protein profiling services. As an innovation orientated CRO company, we constantly develop new methods or improve the existing ones. DigiWest® is one of our proprietary services. We combine innovative thinking with a personal approach to our clients.

Contact us

Email: berlin@nmi-tt.de
Phone: +49 173 751 0585

NMI TT Pharmaservices
Campus Berlin-Buch, D79, 1. OG
Robert-Rössle-Str. 10
13125 Berlin

How it Works

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Pharma Publications

  • Eli Lilly: Blosser et al. A pan-cancer transcriptome analysis identifies replication fork and innate immunity genes as modifiers of response to the CHK1 inhibitor prexasertib. Oncotarget 2020
  • Bayer: Christian et al. The novel dihydroorotate dehydrogenase (DHOH) inhibitor BAY2402234 triggers differentiation and is effective in the treatment of myeloid malignancies. Leukemia 2019
  • Galderma: Mehul et al. Mass spectrometry and DigiWest technology emphasize protein acetylation profile from [...] treated HuT78 CTCL cell line. J of Proteomics 2018
  • Bayer: Kissel et al. Antitumor effects of [...] in preclinical models of hepatocellular carcinoma. Oncotarget 2017


Our Publications

    • With the University of Tübingen: Marzi J, et. al. Noninvasive Physical Plasma as Innovative and Tissue-Preserving Therapy for Women Positive for Cervical Intraepithelial Neoplasia. Cancers (Basel) 2022.
    • With the University of Tübingen: Holl M, et. al. Cell Type-Specific Anti-Adhesion Properties of Peritoneal Cell Treatment with Plasma-Activated Media (PAM). Biomedicines 2022.
    • With the Charles Uni: Skoda J, et. al. Off-target lipid metabolism disruption by the mouse constitutive androstane receptor ligand TCPOBOP in humanized mice. Biochemical Pharmacology 2022.
    • With DKFZ: Bamberg L V, et. al.Targeting euchromatic histone lysine methyltransferasessensitizes colorectal cancer to histone deacetylase inhibitors. Intern J of Cancer 2022.
    • With the NMI: Billing F, et. al. Altered Proinflammatory Responses to Polyelectrolyte Multilayer Coatings Are Associated with Differences in Protein Adsorption and Wettability. Applied Mater Interfaces 2021.
    • With the NMI: Kling S, et. al.Characterization of hepatic zonation in mice by mass-spectrometric and antibody-based proteomics approaches. Bio Chem 2021.
    • With the NMI : Fink S, et. al. Multiplexed Serum Antibody Screening Platform Using Virus Extracts from Endemic Coronaviridae and SARS-CoV-2. ACS Infect Dis 2021.
    • With the University Hospital Münster: Pogatzki-Zahn E, et. al. A proteome signature for acute incisional pain in dorsal root ganglia of mice. Pain 2021.
    • With the University of Tübingen: Zbinden A, et. al. Nidogen-1 Mitigates Ischemia and Promotes Tissue Survival and Regeneration. Advanced Science 2020.
    • With the University of Edinburgh: Shukla R, et. al. Activation of transcription factor circuity in 2i-induced ground state pluripotency is independent of repressive global epigenetic landscapes. Nucleic Acids Research 2020.
    • With Charité Berlin: Bockmayr T, et. al. Multiclass cancer classification in fresh frozen and formalin-fixed paraffin-embedded tissue by DigiWest multiplex protein analysis. Laboratory Investigation 2020.
    • With Freie Universität Berlin: Jevtic M, et. al. Impact of intercellular crosstalk between epidermal keratinocytes and dermal fibroblasts on skin homeostasis. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 2020
    • With BfR: Treindl et al. Array-based Western-blotting reveals spatial differences in hepatic signaling and metabolism following CAR activation. Archives of Toxicology 2020
    • With Trinity College Dublin: Inder et al. Multiplex profiling identifies clinically relevant signaling proteins in an isogenic prostate cancer model of radioresistance. Scientific Reports 2019
    • With the University of Düsseldorf: Naskou et al. EZH2 loss drives resistance to carboplatin and paclitaxel in serous ovarian cancers expressing ATM. Molecular Cancer Research 2019
    • With DKFZ: Zhan et al. MEK inhibitors activate Wnt signaling and induce stem cell plasticity in colorectal cancer. Nature Communications 2019
    • With CELLphenomics: Schumacher et al. Heterogeneous pathway activation and drug response modeled in colorectal-tumor-derived 3d cultures. PLoS Genetics 2019
    • With DKFZ: Augustin et al. Autocrine Wnt regulates the survival and genomic stability of embryonic stem cells. Science Signaling 2017
    • With the University of Göttingen: Menck et al. Neutral sphingomyelinases control extracellular vesicles budding from the plasma membrane. J Extracell Vesicles 2017
    • With the University of Tübingen: Treindl et al. A bead-based western for high-throughput cellular signal transduction analyses. Nature Communications 2016