CRISPR Cell Lines
Based on our long-standing expertise in generating customized stable cell lines, we are now also employing the CRISPR/Cas9 genome editing approach to develop your CRISPR-edited cell models.
Full freedom to operate (FTO) is ensured through a set of license agreements (please see our agreement with ERS Genomics).
Introduction of specifically site-directed double stand breaks applying the CRISPR/Cas9 technology
Generation of target-defective / knockout cell lines
Larger deletions by two double strand breaks
Insertion of specific mutations or extensions into the DNA locus with ectopic repair templates
Generation of synthetic human hiPSC disease models
- CRISPR editing of primary T cells
Beyond the production of custom cell lines, we employ the CRISPR/Cas9 technology also as part of our target validation studies, analogous to RNAi mediated target silencing.
Immunofluorescence image of C-terminal hemagglutinin tagging of endogenous HSP60 in breast carcinoma (A) and HEK293 (B) cells.