CRISPR Cell Lines

Based on our long-standing expertise in generating customized stable cell lines, we are now also employing the CRISPR/Cas9 genome editing approach to develop your CRISPR-edited cell models.

Full freedom to operate (FTO) is ensured through a set of license agreements (please see our agreement with ERS Genomics).

We offer

  • Introduction of specifically site-directed double stand breaks applying the CRISPR/Cas9 technology

  • Generation of target-defective / knockout cell lines

  • Larger deletions by two double strand breaks

  • Insertion of specific mutations or extensions into the DNA locus with ectopic repair templates

  • Generation of synthetic human hiPSC disease models

  • CRISPR editing of primary T cells

 

Beyond the production of custom cell lines, we employ the CRISPR/Cas9 technology also as part of our target validation studies, analogous to RNAi mediated target silencing.

Immunofluorescence image of C-terminal hemagglutinin tagging of endogenous HSP60 in breast carcinoma (A) and HEK293 (B) cells.