Custom Stable Cell Lines

We provide you with customized cell lines expressing your target gene of choice, to support target and biomarker screening in industry and academia. So far, we have generated:

  • 250 cDNA expression vectors,
  • 850 stable recombinant cell lines for the expression of
  • 200 targets, using
  • 150 maternal cell lines or primary cells.
  • Numerous CRISPR-edited cell lines.

Target validation

  • Cloning your gene of interest into a suitable expression vector
  • Selection of promoters of different strengths
  • Lentiviral transduction of a cell line / primary cell type of your choice
  • Selection of stable recombinant cell lines (polyclonal or monoclonal), validated by gene expression analysis at the mRNA / protein level
  • Characterisation with respect to target protein localisation by immunofluorescence microscopy or FACS analysis
  • Full range of functional characterisations (e.g. cell proliferation, induction of apoptosis, metabolism; please inquire for further assays)

High-content analysis of cellular phenotypes

  • Combine the Chromobody┬« technology with your gene of interest!
  • Double expression of your target gene and of an indicative Chromobody┬« in a single cell line
  • High-content analysis for monitoring cell cycle progression, nuclear morphology, cytoskeletal integrity

 

For further information and pricing please contact Dr. Frank Weise.