DigiWest® Protein Profiling Services

Imagine you'd get data from hundreds of western blots in just a couple of days ... Our DigiWest® technology is very close to western blot, but through Luminex® bead-based multiplexing, the system is able to analyze up to 800 total and phospho proteins per sample in one run. Which enables very comprehensive insights on the protein level. Contact us to learn more.

Multiplexed immunoassays for demanding projects

DigiWest® allows studying up to 800 total and phospho proteins in one go, using just 20-60 µg of total protein. Gaining insights into cell signaling, looking at protein signatures for compound characterization, or identifying protein biomarkers - DigiWest® solves complex questions.

Multiplex immunoassay, 80-800 analytes, antibodies, proteins, 20-80 ug of sample, FF or FFPE, pathway panels, MoA, compound characterization, protein biomarker discovery, safety/tox studies, human, mouse, rat, dog, cyano, posttranslational modifications

DigiWest® is flexible to meet your needs

DigiWest® allows protein analyses from 2D or 3D cells, organoids, xenografts, PDX, tissues, fresh-frozen or FFPE sections, and using samples from human, mouse, rat, dog, minipig or cynomolgus origin. Get in contact to discuss the study you have in mind.

Image of DigiWest Protein Profiling in Multiple Species

Cell signaling analyses are no more a struggle

Studying signaling pathways finally becomes fun. Based on 1,200+ antibodies within 50+ pathway panels, it takes just a few clicks to create the custom set of antibodies that is important for your research. And our list includes 300+ antibodies against phospho-epitopes and other post-translational modifications (PTMs). Picking single antibodies or removing a few out of a certain pathway panel is still easy - your selection is fully customizable.

Or try out our Pathway Activity Panels, which were designed to analyse the activation status of the most important cell signaling pathways simultaneously, using pairs of total and phospho antibodies for many of the key proteins (core panel: 90 analytes, extended panel: 184 analytes, complete panel: 242 analytes). Please contact us to learn more.

Case study 1: DigiWest® for compound characterization

Mode-of-action study of lead compounds in comparison to reference drugs

  • Calu1 cells were treated with 1 MEK inhibitor vs 1 PI3K inhibitor vs 2 experimental lead compounds (data not shown) vs DMSO. Cell samples were analysed by DigiWest®, using 156 selected antibodies (total and phospho proteins) covering different signal transduction pathways.

  • DigiWest® yielded distinct signatures for each compound, and allowed in-depth characterization of lead compounds as compared to reference drugs.

Image of DigiWest - Lead Characterization

Case study 2: DigiWest® for biomarker discovery

Protein profiling for characterization of tumor organoid models

  • In collaboration with our partners CELLphenomics GmbH and ASC Oncology GmbH, primary patient-derived 3D tumor organoid cultures (PD3D®) were profiled by DigiWest®, focusing on 122 antibodies for total and phospho proteins involved in a range of signaling pathways.

  • DigiWest® revealed pathway activation patterns that explained phenotypic differences in PD3D® drug responses as measured by CELLphenomics.

Image of DigiWest in PD3D Tumor Cell Models

Like a successful scientist, DigiWest® has a bunch of publications

  • Zhan T et al. MEK inhibitors activate Wnt signaling and induce stem cell plasticity in colorectal cancer. Nature Communications 2019
  • Christian S, et al. The novel dihydroorotate dehydrogenase (DHOH) inhibitor BAY 2402234 triggers differentiation and is effective in the treatment of myeloid malignancies. Leukemia 2019
  • Schumacher D, et al. Heterogeneous pathway activation and drug response modelled in colorectal-tumor-derived 3d cultures. PLoS Genetics 2019
  • Hoerst K, et al. Regenerative potential of adipocytes in hypertrophic scars is mediated by myofibroblast reprogramming. J Mol Med 2019
  • Sikorski K, et al. A high-throughput pipeline for validation of antibodies. Nature Methods 2018
  • Mehul B, et al. Mass spectrometry and DigiWest technology emphasize protein acetylation profile from [...] treated HuT78 CTCL cell line. Journal of Proteomics 2018
  • Lomnytska M, et al. Platelet protein biomarker panel for ovarian cancer diagnosis. Biomarker Research 2018
  • De Boussac H, et al. Epidermal Growth Factor represses CAR expression in primary hepatocytes and favors regulation by PXR. Drug Metab Dispos 2017
  • Kissel M, et al. Antitumor effects of [...] in preclinical models of hepatocellular carcinoma. Oncotarget 2017
  • Mishra M, et al. Protein purification and analysis: next generation Western blotting techniques. Expert Rev Proteomics 2017
  • Menck K, et al. Neutral sphingomyelinases control extracellular vesicles budding from the plasma membrane. J Extracell Vesicles 2017
  • Augustin I, et al. Autocrine Wnt regulates the survival and genomic stability of embryonic stem cells. Science Signaling 2017
  • Treindl F, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nature Communications 2016

Other references

  • DigiWest: Multiplex profiling of proteins for biomarker discovery. LabsExplorer 2018
  • Sachse C, Templin M. Multiplexing Western blots: More data, less sample. GEN 2018
  • Erdmann G, et al. Towards 3Rs: Multiplex protein profiling from minipig, cyno, dog and rat samples, to get the most our of preclinical safety studies. Ellegaard Newsletter 2018
  • Graham H. Drop the blot: Parallel quantification of up to 800 proteins. Luminex Blog 2018
  • Singh G. The future of now - how the science of today shapes tomorrow. ScienceExchange Blog 2018
  • Pearson S. Screening innovations and 3D assay developments showcased at ELRIG. GEN 2017
  • Inder S, et al. Profiling of radioresistant prostate cancer cells identifies key protein. BJUI 2017
  • Sachse C, Regenbrecht CRA, Templin MF. Beyond Genomics. DigiWest-Technologie: Multiplex-Analyse von Proteinen und Phosphoproteinen. LABO 2017
  • How to find the missing link between genotype and phenotype. Labiotech 2017
  • Evans J. A blot on the beads: Western blotting with a bead-based immunoassay. Electrophoresis 2016
  • Couvillon A, Templin MF. Cross-validation of antibodies using DigiWest. LabRoots 2016
  • Graham H. DigiWest: Reimaging Western blotting on the proteomic scale. Luminex 2016