hiPSC Disease Modeling
Building on the recent advances in working with human induced pluripotent stem cell systems, we have established a versatile portfolio of services for generation of hiPSC disease models.
Sources of hiPSC-derived disease models
Biopsy material (e.g. skin) from clinically diagnosed individuals for generation of hiPSCs and derived cell types
Benefit from genetic backgrounds of individual patients
Identify and validate disease-causing targets and mechanisms
Genome editing (CRISPR/Cas9) in established hiPSC lines to generate synthetic human disease models
Study defined disease-causing mutations or alleles
Ensure enhanced comparability of observed phenotypes through isogenic background of cellular models
Phenotypic analyses of hiPSC-derived disease models
ICC workflows for single cell analysis of synaptic connectivity
HCA routines to monitor neuronal development (e.g. neurite outgrowth)
Electrophysiological and live cell imaging (e.g. Ca2+-sensitive dyes) recordings to evaluate parameters of neuronal activity
RT-qPCR panels (QuantStudio™ 12K Flex) to determine model-specific gene expression signatures
Grunwald, Lena-Marie, et al. "Comparative characterization of human-induced pluripotent stem cells (hiPSC) derived from patients with schizophrenia and autism." Translational psychiatry 9.1 (2019): 179.