Gene Modulation by shRNAs and siRNAs
We employ inter alia lentiviral vectors to down-regulate the gene expression of target genes via shRNA-mediated RNA interference (RNAi). Alternatively, we use commercially available siRNAs for cell-based RNAi gene silencing experiments.
Generation of lentivirus encoding target-specific shRNAs
Production of stable knock-down cell lines
Validation of target silencing by RT-qPCR, Western blot and/or immunofluorescence microscopy
RNAi studies with shRNAs or siRNAs, using a wide range of reader-based and fixed endpoint or live microscopy assays
A further expansion of possibilities is provided by the CRISPR/Cas9 technology, which we employ to abrogate target expression.